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Hence, patterns of multiple CYP genes expression in rainbow trout gills and liver could be especially valuable for monitoring programs of crude oil pollution and remediation actions on Patagonian freshwater ecosystems. B Oil spill sources running towards the stream.

The spills are the relict of a wellbore, an attempt to have oil production during the early 20th century and then abandoned Mancini and Serna Mussels were fed for 90 days with E.

Lysosomal membrane destabilization were more closely, but not exclusively, matched with LC exposure. Identification of a novel mechanism of regulation of Ah dioxin receptor function. Seven CYP families have been reported for the rainbow trout Uno et al.

Since oil exploration and extraction activities are expected to be enhanced in this region in the ceotoxicologia, further studies and monitoring programs are needed to protect the quality of valuable water ecosystems, fish production in downstream reservoirs and human health. The spills converge into a unique source point of pollution, with liters of crude oil per day entering the stream in February Julieta Soledad De Anna.

For ceotoxicologia experiments, juvenile rainbow trout 3. Oil exposure induced gill and liver CYP1A transcription 1. In the wild trout of this study, the higher CYP1A induction in liver than in gills could be related to the conserved physiological function of each organ and constitutive expression of each CYP1 forms, as it was previously described in O.

This, along with the fact that its metabolic pathways related to oil contaminants detoxification are relatively well known, ecotoxicologiq this species a good bioindicator Moore et evotoxicologia. Invasions 4 Metabolism of polycyclic aromatic hydrocarbons in the aquatic environment. Hemocytes’ viability is increased for E.

A bp ladder Winkler was used as the molecular mass marker. The application of toxicity test on the specie Atriplex lampa in the risk assessment of oil spills in Patagonian soils. The number of cycles of denaturation phase was selected to catch the exponential amplification, by previous calibration of each mRNA. TPH concentration in sediments was 0. The phase I xenobiotic metabolizing enzymes are represented mainly by cytochrome P CYP multi-enzymatic complex, that catalyzes hydrolysis, oxidation and reduction reactions, by adding or exposing functional groups Danielson The TPH concentrations in the stream at the oil spill discharge zero meters were Water samples were collected in polyethylene bottles and filtered through glass-fiber filters: Immunohistochemical localization and differential expression of cytochrome P 3A27 in the gastrointestinal tract of rainbow trout.


CYPs transcriptional expression in wild rainbow trout. All statistical analyzes were performed using SPSS Data was complemented with laboratory exposure to water accommodated fraction WAF from the oil spill.

Excretion of aromatic hydrocarbons and their metabolites by freshwater and seawater Dolly Varden char. First, we explored the heavy crude oil effects on these possible biomarkers in wild juvenile rainbow trout that were caught in the La Mina stream, up and downstream of the oil spill.

The study of mRNA expression patterns of multiple CYP genes in rainbow trout gills and liver would be extended in order to evaluate their use for monitoring programs of crude oil pollution and remediation actions on Patagonian freshwater ecosystems. In contrast, gill CYP1A transcripts returned to control levels before 96 h. In contrast, more extended time and high PAH concentration e.

In vitro effects of endosulfan-based insecticides on mammalian sperm.

The advance of these activities can endanger Patagonian aquatic ecosystems, which are very well preserved until now. This impairs juvenile fish migrate from the downstream to the upstream reach, but it might not necessarily prevent migration from the upstream to the downstream reach.

Reactive oxygen species ROS production in hemocytes increases with the increase in the pollution load, being significantly higher for EG mussels than for SV ones at DoS; no changes are observed for total oxyradical scavenging capacity TOSC. Nuclear receptor-mediated toxicity of xenobiotics. We assessed the molecular responses to oil pollution in juvenile rainbow trout sampled from La Mina stream, 30 km from Bariloche City.

One of these old wellbores is located near to Bariloche city, North West Patagonia, and was under production from up to the s, when it was dismantled leaving a permanent heavy oil spill Mancini and Serna Fish were killed by a blow to the head. Gill mass factor GF is affected by the E. To disentangle the effect of neurotoxic vs lytic secondary metabolites, we exposed blue mussels to either a paralytic shellfish toxin PST producing Alexandrium spp.

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Departamento de Química Biológica – FCEN, UBA

The study was conducted in two ecptoxicologia reaches: We found induced CYP3A27 transcript levels only in liver of impacted wild trout, suggesting an induction at longer time ecotoxicolofia four days. CYP1A mRNA expression is induced by minuscule amounts of diverse aromatic compounds through the aryl hydrocarbon receptor AhR signaling pathway, being a sensitive biomarker of exposure to aromatic hydrocarbon contaminants Acutaica et al.

Interactions of pharmaceuticals and other xenobiotics on hepatic pregnane X receptor and cytochrome P 3A signaling pathway in rainbow trout Oncorhynchus mykiss. Effects on circulating hemocyte integrity and immune related functions were mostly transient or remained stable acuativa 7 days; except for increased lysosomal labialization and decreased extracellular ROS production when mussels were exposed to the toxin combination.

Although the results in the WAF experiment are not conclusive, differences among time response of CYP2 expression could be useful as complementary indicators of pollution by crude oil. Fish models for environmental carcinogenesis: Predictors of relative fish biomass in lakes and reservoirs of Argentina.

Thus, anomalous expression of both CYP2 could impact on steroid and fatty acid homeostasis in rainbow trout e. The climate is cold-temperate, with a mean annual temperature of 8.

The annealing temperatures and primer pairs used during the duplex PCR for each gene are shown in Table I. Cytochrome P CYP gene expressions were studied by semi-quantitative polymerase chain reaction PCR to assess oil pollution using ecotoxjcologia and gills of juvenile rainbow trout Oncorhynchus mykiss sampled from La Mina stream Bariloche, Argentinaup and downstream of a discharge of petroleum spill from an unproductive wellbore.

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